ELUXI_Ind_Patern_Illumination

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Ind Collimated LEDs Ind Fibre Coupled LEDs Ind LED Spots Ind Lightguide Coupled LEDs Ind Multi Wavelength Ind Patern Illumination Ind Precision LED Spots

Together with its partners ELUXI is able to offer a comprehensive range of solutions in this area. If you cannot find what you are looking for from the information below, please contact us directly.

PATERN ILLUMINATION

INDUSTRIAL LED SOURCES

Multi Wavelength Pattern Illuminator on Inverted Microscope

Features

Programmable image patterns
Up to 4000 frames per second
416K pixel DLP panel
Wide range of available wavelengths
Support LEDs or arc lamps
Diffraction-limited projection
High-throughput Etendue preserving design
External LED controller for fast wavelength switching
Fiber or lightguide input available
Intuitive software for spatial, temporal and spectral control of illumination patterns

Spatially patterned illumination with temporal and spectral control enables numerous new techniques in life-science applications such as optogenetics. For example, in optogenetics, selected neurons in a specimen slice can be activated or silenced with a user defined illumination pattern.

Mightex Polygon400 multi-wavelength dynamic spatial illuminator (DSI) integrates the state-of-theart spatial light modulators and high-power LEDs using a proprietary Etendue-preserving optical design to deliver high-intensity illumination patterns with diffraction-limited resolution.

A Texas Instruments’ DLP spatial light modulator is used to display a user defined image pattern. At the heart of the Polygon400 is a unique optical system that carefully delivers light from LED sources to the DLP panel and then through a microscope to the specimen plane. Such a systematic approach makes it possible to achieve maximum optical intensity while maintaining diffraction-limited imaging performance.

Temporal performance is a key to many intended applications for Polygon400. With a frame rate of more than 4,000 fps and fast-switching LEDs, Mightex Polygon400 can deliver illumination patterns with micro-second precision. Advanced DSI models with external LED controllers also enable wavelength switching in between illumination patterns at the highest frame rate.

A dedicated software allows users to generate illumination patterns as well as control illumination intensity and timing. The software also supports alignment between illumination patterns and images acquired through any digital cameras on a microscope.

Polygon400 is designed to be easily inserted into the infinity path of a microscope. For inverted microscopes, the preferred inserting point is usually the back port of the microscope where a fluorescence attachment is commonly placed. A filter cube is required to fold the DSI light path into the microscope. The filter cubes used for fluorescence observation serves this purpose well. For upright microscopes we provide a beam combiner cube to be inserted below the binocular/trinocular

unit. The dichroic or mirror in the beam combiner directs the Polygon400 beam into the microscope light path.

Customers may specify up to three LEDs in the range of 400nm to 700nm to be included in the built-in light source engine. A lightguide-coupled model Polygon400g is also available where any light sources such as LEDs and arc lamps can be used for illumination. Note that the lightguidecoupled model does not have built-in LEDs.

The “E” version of Polygon400 bypasses the internal LED controller and allows users to directly control the built-in LEDs through external LED drivers. The cathodes and anodes of built-in LEDs are connected to a multi-pin connector on the back panel of Polygon400.

External LED controllers enable several important features not supported by the internal LED controller.

One can achieve higher dynamic range, higher intensity resolution(>10bits), higher peak current in pulse mode, and faster response(<10ns).

The use of external LED controllers also makes it possible to control LED timing independent of the micromirror array. For example, while the micromirrors stay at a constant position, the LEDs can be modulated to generate desired intensity profile. On the other hand, when needed, strict synchronization between the LEDs and the micromirrors can be achieved via the external trigger signals provided by the Polygon400.

Mightex supplies a variety of LED controllers that can be used with the “E” version of Polygon400. Polygon400 software already includes synchronization features to support Mightex BLS- series LED controllers. For third-party LED controllers, synchronization can be implemented via trigger signals or Polygon400 SDK.