Multi Wavelength Pattern Illumination
Spatially patterned illumination with temporal and spectral control enables numerous new techniques in life-
Mightex Polygon400 multi-
A Texas Instruments’ DLP spatial light modulator is used to display a user defined image pattern. At the heart of the Polygon400 is a unique optical system that carefully delivers light from LED sources to the DLP panel and then through a microscope to the specimen plane. Such a systematic approach makes it possible to achieve maximum optical intensity while maintaining diffraction-
Temporal performance is a key to many intended applications for Polygon400. With a frame rate of more than 4,000 fps and fast-
A dedicated software allows users to generate illumination patterns as well as control illumination intensity and timing. The software also supports alignment between illumination patterns and images acquired through any digital cameras on a microscope.
Polygon400 is designed to be easily inserted into the infinity path of a microscope. For inverted microscopes, the preferred inserting point is usually the back port of the microscope where a fluorescence attachment is commonly placed. A filter cube is required to fold the DSI light path into the microscope. The filter cubes used for fluorescence observation serves this purpose well. For upright microscopes we provide a beam combiner cube to be inserted below the binocular/trinocular
unit. The dichroic or mirror in the beam combiner directs the Polygon400 beam into the microscope light path.
Customers may specify up to three LEDs in the range of 400nm to 700nm to be included in the built-
The “E” version of Polygon400 bypasses the internal LED controller and allows users to directly control the built-
External LED controllers enable several important features not supported by the internal LED controller.
One can achieve higher dynamic range, higher intensity resolution(>10bits), higher peak current in pulse mode, and faster response(<10ns).
The use of external LED controllers also makes it possible to control LED timing independent of the micromirror array. For example, while the micromirrors stay at a constant position, the LEDs can be modulated to generate desired intensity profile. On the other hand, when needed, strict synchronization between the LEDs and the micromirrors can be achieved via the external trigger signals provided by the Polygon400.
Mightex supplies a variety of LED controllers that can be used with the “E” version of Polygon400. Polygon400 software already includes synchronization features to support Mightex BLS-